International Drug Collection Drug repositioning approaches for the discovery of new therapeutics for Alzheimer's disease. Tae-Wan Kim. Neurotherapeutics 12:132 (2015).

The approved pediatric drug suramin identified as a clinical candidate for the treatment of EV71 infection— suramin inhibits EV71 infection in vitro and in vivo. Peijun Ren, Gang Zou, Benjamin Bailly, Shanshan Xu, Mei Zeng, Xinsheng Chen, Liang Shen, Ying Zhang, Patrice Guillon, Fernando Arenzana-Seisdedos, Philippe Buchy, Jian Li, Mark von Itzstein, Qihan Li and Ralf Altmeyer.Emerging Microbes & Infections 3:e62 (2014).

NatProd CollectionPodophyllotoxin acetate triggers anticancer effects against non-small cell lung cancer cells by promoting cell death via cell cycle arrest, ER stress and autophagy. Jae Yeon Choi, Wan Gi Hong, Jeong Hyun Cho, Eun Mi Kim, Jongdoo Kim, Chan-Hun Jung, Sang-Gu Hwang, Hong-Duck Um and Jong Kuk Park. Int J Oncol 47:1257 (2015).

Computational fishing of new DNA methyltransferase inhibitors from natural products. Wilson Maldonado-Rojas, Jesus Olivero-Verbel and Yovani Marrero-Ponce. J Mol Graphics Modeling 60:43 (2015).

Podophyllotoxin acetate enhances γ-ionizing radiation-induced apoptotic cell death by stimulating the ROS/p38/caspase pathway. Jae Yeon Choi, Hyun-Ji Cho, Sang-Gu Hwang, Wun-Jae Kim, Jong-Il Kim, Hong-Duck Um and Jong Kuk Park. Biomed Pharmacol 70:111 (2015).

Zebrafish-based small molecule screens for novel cardiovascular drugs. Peter Novodvorsky, Marc MJ Da Costa and Timothy JA Chico. Drug Discovery Today. Technologies:10:e109 (2013).

High-throughput library screening identifies two novel NQO1 inducers in human lung cells, Xiang-Lin Tan, Gaby Marquardt, Aldo B Massimi, Miao Shi, Weiguo Han, and Simon D Spivack. Am J Respir Cell Mol Biol 46:365 (2012).

Limonoid compounds inhibit sphingomyelin biosynthesis by preventing cert protein-dependent extraction of ceramides from the endoplasmic reticulum. Françoise Hullin-Matsuda, Nario Tomishige, Shota Sakai, Reiko Ishitsuka, Kumiko Ishii, Asami Makino, Peter Greimel, Mitsuhiro Abe, Elad L Laviad, Michel Lagarde, Hubert Vidal, Tamio Saito, Hiroyuki Osada, Kentaro Hanada, Anthony H Futerman and Toshihide Kobayashi. J Biol Chem 287:24397 (2012).

The anticholinesterase phenserine and its enantiomer posiphen as 5′untranslated-region-directed translation blockers of the parkinson's alpha synuclein expression. Sohan Mikkilineni, Ippolita Cantuti-Castelvetri, Catherine M Cahill, Amelie Balliedier, Nigel H Greig and Jack T Rogers. Parkinson's Disease 2012:ID142372 (2012)

Disease allele-dependent small-molecule sensitivities in blood cells from monogenic diabetes. Stanley Y. Shaw, David M Blodgett, Maggie S Ma, Elizabeth C. Westly, Paul A Clemons, Aravind Subramanian and Stuart L Schreiber. PNAS 108:492 (2011).

Validating new tuberculosis computational models with public whole cell screening aerobic activity datasets. Sean Ekins and Joel S Freundlich. Pharm Res 28:1859 (2011).

The Pharmakon Collection Artemisinins, new miconazole potentiators resulting in increased activity against Candida albicans biofilms. Kaat De Cremer, Ellen Lanckacker, Tanne L Cools, Marijke Bax, Katrijn De Brucker, Paul Cos, Bruno PA Cammue and Karin Thevissen. Antimicrob Agents Chemother 59:421 (2015). Antagonism screen for inhibitors of bacterial cell wall biogenesis uncovers an inhibitor of undecaprenyl diphosphate synthase. Maya A Farha, Tomasz L Czarny, Cullen L Myers, Liam J Worrall, Shawn French, Deborah G Conrady, Yang Wang, Eric Oldfield, Natalie CJ Strynadka and Eric D Brown. PNAS 112:11048 (2015).

Inhibiting an epoxide hydrolase virulence factor from Pseudomonas aeruginosa protects CFTR. Christopher D Bahl, Kelli L Hvorecny, Jennifer M Bomberger, Bruce A Stanton, Bruce D Hammock, Christophe Morisseau and Dean R Madden. Angewandte Chem 54:9881 (2015).

In vitro screening for drug repositioning. Graeme F Wilkinson and Kevin Pritchard. J Biomol Screen 20:167 (2015).

Detection of cell aggregation and altered cell viability by automated label-free video microscopy; a promising alternative to endpoint viability assays in high-throughput screening. Obaid Aftab, Mårten Fryknäs, Ulf Hammerling, Rolf Larsson and Mats G Gustafsson. J Biomol Screen 20:372 (2015).

High-throughput luminescent reporter of insulin secretion for discovering regulators of pancreatic beta-cell function. Sean M Burns, Amedeo Vetere, Deepika Walpita, Vlado Dančík, Carol Khodier, Jose Perez, Paul A Clemons, Bridget K Wagner and David Altshuler. Cell Metabolism 21:126 (2015)

The technology development studio of the mpi-cbg: an open access cell-based screening facility. Marc Bickle. Chem High Throughput Screen. 17:322 (2014)

Establishment of a novel whole animal hts technology platform for melioidosis drug discovery Umayal Lakshmanan, Amelia Yap, Justina Fulwood, Li Yichun, Sim Siew Hoon, Jolander Lim, Audrey Ting, Xiao Hui Sem, Jason F Kreisberg, Patrick Tan, Gladys Tan and Horst Flotow.Combinatorial Chem High Throughput Screen. 17:790 (2014).

Repurposing as a means to increase the activity of amphotericin B and caspofungin against Candida albicans biofilms. Nicolas Delattin, Katrijn De Brucker, Katleen Vandamme, Els Meert, Arnaud Marchand, Patrick Chaltin, Bruno P. A. Cammue and Karin Thevissen. J Antimicrob Chemother 69:1035 (2014).

Angiotensin II type 1 receptor blockers increase tolerance of cells to copper and cisplatin Pieter Spincemaille, Gursimran Chandhok, Andree Zibert, Hartmut Schmidt, Jef Verbeek, Patrick Chaltin, Bruno PA Cammue, David Cassiman and Karin Thevissen. Microbial Cell 1:352 (2014).

Dyclonine rescues frataxin deficiency in animal models and buccal cells of patients with Friedreich's ataxia. Sunil Sahdeo, Brian D Scott, Marissa Z McMackin, Mittal Jasoliya, Brandon Brown, Heike Wulff, Susan L Perlman, Mark A Pook and Gino A Cortopassi. Hum Mol Genet 23:6848 (2015).

High-throughput screening of FDA-approved drugs using oxygen biosensor plates reveals secondary mitofunctional effects. Sunil Sahdeo, Alexey Tomilov, Kelly Komachi, Christine Iwahashi, Sandipan Datta, Owen Hughes, Paul Hagerman and Gino Cortopassi. Mitochondrion 17:116 (2014).

Intracellular calcium regulates nonsense-mediated mRNA decay. Andrew Nickless, Erin Jackson, Jayne Marasa, Patrick Nugent, Robert W Mercer, David Piwnica-Worms and Zhongsheng You. Nature Medicine 20:961 (2014).

Development of an HTS assay for EPHX2 phosphatase activity and screening of nontargeted libraries. Christophe Morisseau, Sunil Sahdeo, Gino Cortopass and Bruce D Hammock. Analyt Biochem 234:105 (2013).

Repositioning of the anthelmintic drug mebendazole for the treatment for colon cancer. Peter Nygren,, Mårten Fryknäs,, Bengt Ågerup and Rolf Larsson. J Cancer Res Clin Oncol 139:2133 (2013).

Inhibition of soluble epoxide hydrolase by fulvestrant and sulfoxides. Christophe Morisseau, Svitlana Pakhomova, Sung Hee Hwang, Marcia E Newcomer and Bruce D Hammock. Bioorg Med Chem Lett 23:3818 (2013).

The Spectrum CollectionNovel chemical library screen identifies naturally occurring plant products that specifically disrupt glioblastoma-endothelial cell interactions. Rajarshi Sengupta, Amy Barone, Jayne Marasa, Sara Taylor, Erin Jackson, Nicole M. Warrington, Shyam Rao, Albert H Kim, Jeffrey R Leonard, David Piwnica-Worms and Joshua B Rubin. Oncotarget 6,21:18282 (2015).

High-throughput minigenome system for identifying small-molecule inhibitors of ebola virus replication. Megan R Edwards, Colette Pietzsch, Thibaut Vausselin, Megan L Shaw, Alexander Bukreyev, and Christopher F Basler. ACS Infect Dis 1:380 (2015).

Discovery of bacterial fatty acid synthase type II inhibitors using a novel cellular bioluminescent reporter assay. Joselynn Wallace, Nicholas O Bowlin, Debra M Mills, Panatda Saenkham, Steven M Kwasny, Timothy J Opperman, John D Williams, Charles O Rock,Terry L Bowlin and Donald T Moir. Antimicrob Agents Chemother 59:5775 (2015).

Development and application of a high-throughput screening method to evaluate antifungal activity against Trichophyton tonsurans. Barry Preuett, J Steven Leeder and Susan Abdel-Rahman. J Biomol Chem 20:1171 (2015)

Salt-sparing diuretic action of a water-soluble urea analog inhibitor of urea transporters UT-A and UT-B in rats. Onur Cil, Cristina Esteva-Font, Sadik Taskin Tas, Tao Su, Sujin Lee, Marc O Anderson, Mert Ertunc and Alan S Verkman. Kidney International 88:311 (2015).

Drug repositioning approaches for the discovery of new therapeutics for Alzheimer's disease. Tae-Wan Kim. Neurotherapeutics 12:132 (2015).

Interaction of Paroxetine with Mitochondrial Proteins Mediates Neuroprotection. Joseph P Steiner, Muznabanu Bachani, Brett Wolfson-Stofko, Myoung-Hwa Lee, Tonguang Wang, Guanhan Li, Wenxue Li, David Strayer, Norman J Haughey and Avindra Nath. Neurotherapeutics 12:132 (2015).

Antihelminthic benzimidazoles are novel hif activators that prevent oxidative neuronal death via binding to tubulin. Hossein Aleyasin, Saravanan S Karuppagounder, Amit Kumar, Sama Sleiman, Manuela Basso, Thong Ma, Ambreena Siddiq, Shankar J Chinta, Camille Brochier, Brett Langley, Renee Haskew-Layton, Susan L Bane, Gregory J Riggins, Irina Gazaryan, Anatoly A Starkov, Julie K Andersen and Rajiv R Ratan. Antioxidants Redox Signaling 22:121 (2015).

15 Years of zebrafish chemical screening. Andrew J Rennekamp and Randall T Peterson. Current Opin Chem Biol 24:58 (2015).

Development and validation of a thallium flux-based functional assay for the sodium channel NaV1.7 and its utility for lead discovery and compound profiling. Yu Du, Emily Days, Ian Romaine, Kris K Abney, Kristian Kaufmann, Gary Sulikowski, Shaun Stauffer, Craig W Lindsley, and C David Weaver. ACS Chem Neurosci 6:871 (2015).

A screen of approved drugs identifies the androgen receptor antagonist flutamide and its pharmacologically active metabolite 2-hydroxy-flutamide as heterotropic activators of cytochrome P450 3A in vitro and in vivo. AL Blobaum, FW Byers, TM Bridges, CW Locuson, PJ Conn, CW Lindsley and JS Daniels. Drug Metab Dispos 43:1718 (2015).

Microplate-based assay for identifying small molecules that bind a specific intersubunit interface within the assembled HIV-1 capsid. Upul D Halambage, Jason P Wong, Bruce J Melancon, Craig W Lindsley and Christopher Aiken. .Antimicrob Agent Chemother 59:5190 (2015).

Discovery and development of DNA methyltransferase inhibitors using in silico approaches This paper is dedicated to the memory of Dr Eugene Athanas Bratoeff Titeff honoring his many contributions to drug discovery. José L Medina-Franco, Oscar Méndez-Lucio, Alfonso Dueñas-González and Jakyung Yoo. Drug Discovery Today 20:569 (2015).

Gossypol acetic acid prevents oxidative stress-induced retinal pigment epithelial necrosis by regulating the FoxO3/Sestrin2 pathway. Jakub Hanus, Hongmei Zhang, David H Chen, Qinbo Zhou, Peng Jin, Qinghua Liu and Shusheng Wang. Mol Cell Biol 35:1952 (2015). 4-Acetoxyphenol prevents RPE oxidative stress–induced necrosis by functioning as an NRF2 stabilizer. Jakub Hanus; Alexander Kolkin; Julia Chimienti; Sara Botsay; Shusheng Wang. Invesigat Opthamol Visual Sci 56:5048 (2015).

Dihydrotanshinone-I interferes with the RNA-binding activity of HuR affecting its post-transcriptional function. Vito Giuseppe D'Agostino, Preet Lal, Barbara Mantelli, Christopher Tiedje, Chiara Zucal, Natthakan Thongon, Matthias Gaestel, Elisa Latorre, Luciana Marinelli, Pierfausto Seneci, Marialaura Amadio and Alessandro Provenzani. Sci Reports 5:16478 (2015).

A High-content screen identifies compounds promoting the neuronal differentiation and the midbrain dopamine neuron specification of human neural progenitor cells. Ji heon Rhim, Xiangjian Luo, Xiaoyun Xu, Dongbing Gao, Tieling Zhou, Fuhai Li, Lidong Qin, Ping Wang, Xiaofeng Xia and Stephen TC Wong. Sci Reports 5:16237 (2015).

The therapeutic potential of cystathionine β-synthetase/hydrogen sulfide inhibition in cancer Mark R Hellmich, Ciro Coletta, Celia Chao and Csaba Szabo. Antioxid Redox Signal 22:424 (2015).

A rapid and high content assay that measures cyto-ID-stained autophagic compartments and estimates autophagy flux with potential clinical applications. Sujuan Guo, Yanping Liang, Susan F Murphy, Angela Huang, Haihong Shen, Deborah F Kelly, Pablo Sobrado and Zhi Sheng. Autophagy 11:560 (2015).

Stroke. Strategy for identifying repurposed drugs for the treatment of cerebral cavernous malformation. Christopher C Gibson, Weiquan Zhu, Chadwick T Davis, Jay A Bowman-Kirigin, Aubrey C Chan, Jing Ling, Ashley E Walker, Luca Goitre, Simona Delle Monache, Saverio Francesco Retta, Yan-Ting E Shiu, Allie H Grossmann, Kirk R Thomas, Anthony J Donato, Lisa A Lesniewski, Kevin J Whitehead and Dean Y Li. Circulation 131:289 (2015).

Screening for Anti-cancer Drugs in Drosophila. Helena E Richardson, Lee Willoughby and Patrick O Humbert. eLS 2015:1.

6-Phosphogluconate dehydrogenase links oxidative PPP, lipogenesis and tumour growth by inhibiting LKB1–AMPK signalling. Ruiting Lin, Shannon Elf, Changliang Shan, Hee-Bum Kang, Quanjiang Ji, Lu Zhou, Taro Hitosugi, Liang Zhang, Shuai Zhang, Jae Ho Seo, Jianxin Xie, Meghan Tucker, Ting-Lei Gu, Jessica Sudderth, Lei Jiang, Matthew Mitsche, Ralph J DeBerardinis, Shaoxiong Wu, Yuancheng Li, Hui Mao, Peng R Chen, Dongsheng Wang, Georgia Zhuo Chen, Selwyn J Hurwitz, Sagar Lonial, Martha L Arellano, Hanna J Khoury, Fadlo R Khuri, Benjamin H Lee, Qunying Lei, Daniel J Brat, Keqiang Ye, Titus J Boggon, Chuan He, Sumin Kang, Jun Fan, Jing Chen. Nature Cell Biol 17:1484 (2015).

US Drug CollectionNuclear receptor Nurr1 agonists enhance its dual functions and improve behavioral deficits in an animal model of Parkinson's disease. Chun-Hyung Kim, Baek-Soo Han, Jisook Moon, Deog-Joong Kim, Joon Shin, Sreekanth Rajan, Quoc Toan Nguyen, Mijin Sohn, Won-Gon Kim, Minjoon Han, Inhye Jeong, Kyoung-Shim Kim, Eun-Hye Lee, Yupeng Tu, Jacqueline L. Naffin-Olivos, Chang-Hwan Park, Dagmar Ringe, Ho Sup Yoon, Gregory A. Petsko and Kwang-Soo Kim. Proc Nat Acad Sci 112:8756 (2015)

Testing of Middle East respiratory syndrome coronavirus replication inhibitors for the ability to block viral entry. Qi Liu, Shuai Xia, Zhiwu Sun, Qian Wang, Lanying Du, Lu Lu and Shibo Jiang. Antimicrob Agents Chemother 59:742 (2015).

Identification of nitazoxanide as a group I metabotropic glutamate receptor negative modulator for the treatment of neuropathic pain: an in silico drug repositioning study. Ni Ai, Richard D Wood and , William J. Welsh. Pharm Res 32:2798 (2015)

Drug repositioning approaches for the discovery of new therapeutics for Alzheimer's disease. Tae-Wan Kim. Neurotherapeutics 12:132 (2015).

Identification of new inhibitors for human hematopoietic prostaglandin D2 synthase among FDA-approved drugs and other compounds. Aslam M.A. Mazari, Usama M. Hegazy and Bengt Mannervik. Chemico-Biolog Interactions. 229:91 (2015).

Novel therapeutic strategy for neurodegeneration by blocking Aβ seeding mediated aggregation in models of Alzheimer's disease. Simona Eleuteri, Saviana Di Giovanni, Edward Rockenstein, Mike Mante, Antony Adame, Margarita Trejo, Wolf Wrasidlo, Fang Wu, Patrick C Fraering, Eliezer Masliah and Hilal A. Lashuel. Neurobiol Disease 74:144 (2015).

The approved pediatric drug suramin identified as a clinical candidate for the treatment of EV71 infection— suramin inhibits EV71 infection in vitro and in vivo. Peijun Ren, Gang Zou, Benjamin Bailly, Shanshan Xu, Mei Zeng, Xinsheng Chen, Liang Shen, Ying Zhang, Patrice Guillon, Fernando Arenzana-Seisdedos, Philippe Buchy, Jian Li, Mark von Itzstein, Qihan Li and Ralf Altmeyer.Emerging Microbes & Infections 3:e62 (2014).

Phenotypic screens targeting neurodegenerative diseases. Minhua Zhang, Guangrui Luo, Yanjiao Zhou, Shaohui Wang and Zhong Zhong.J Biomol Screen 19:11 (2014).

Drosophila modifier screens to identify novel neuropsychiatric drugs including aminergic agents for the possible treatment of Parkinson's disease and depression. H O Lawal, A Terrell, H A Lam, C Djapri, J Jang, R Hadi, L Roberts, V Shahi, M-T Chou, T Biedermann, B Huang, G M Lawless, N T Maidment and D E Krantz. Molecular Psychiatry 19:235 (2014)

Broad-spectrum antivirals for the emerging Middle East respiratory syndrome coronavirus. Jasper FW Chan, Kwok-Hung Chan, Richard YT Kao, Kelvin KW To, Bo-Jian Zheng, Clara PY Li, Patrick TW Li, Jun Dai, Florence KY Mok, Honglin Chen, Frederick G Hayden and Kwok-Yung Yuen. J Infection 67:606 (2013). Drug library screening against metronidazole-sensitive and metronidazole-resistant Trichomonas vaginalis isolates. E Brook Goodhew and W Evan Secor. Sex Transm Infect 89:479 (2013).

Cancer cell senescence: a new frontier in drug development. Claire J Cairney, Alan E Bilsland, TR Jeffry Evans, Jon Roffey, Dorothy C Bennett, Masashi Narita, Christopher J Torrance and W Nicol Keith. Drug Discovery Today 17:269 (2012).

Antineoplastic activity of ouabain and pyrithione zinc in acute myeloid leukemia. M Tailler, L Senovilla, E Lainey, S Thépot, D Métivier, M Sébert, V Baud, K Billot, P Fenaux, L Galluzzi, S Boehrer, G Kroemer and O Kepp. Oncogene 31:3536 (2012).

A high-content screening assay in transgenic zebrafish identifies two novel activators of fgf signaling. M Saydmohammed, LL Vollmer, EO Onuoha, A Vogt and M Tsang, Birth Defects Research Part C: Embryo Today: Reviews, 93:281 (2011). Harmine, a natural beta-carboline alkaloid, upregulates astroglial glutamate transporter expression. Yun Li, Rita Sattler, Eun Ju Yang, Alice Nunes, Yoko Ayukawa, Sadia Akhtar, Grace Ji, Ping-Wu Zhang and Jeffrey D Rothstein. Neuropharmacol 60:1168 (2011). Optimization of propafenone analogues as antimalarial leads. David J Lowes, W Armand Guiguemde, Michele C Connelly, Fangyi Zhu, Martina S Sigal, Julie A Clark, Andrew S Lemoff, Joseph L Derisi, Emily B Wilson and R Kiplin Guy. J Med Chem 54:7477 (2011).

Dual-fluorescence isogenic high-content screening for MUC16/CA125 selective agents. Thapi D Rao, Nestor Rosales and David R Spriggs. Mol Cancer Therap 10:1939 (2011).

The Killer Plates Celastrol inhibits Plasmodium falciparum enoyl-acyl carrier protein reductase. Lorillee Tallorin, Jacob D Durrant, Quynh G Nguyen, J Andrew McCammon and Michael D Burkart. Bioorg Med Chem 22:6053 (2014).

Drug repurposing and human parasitic protozoan diseases. Katherine T Andrews, , Gillian Fisher and Tina S Skinner-Adams. Int J Parasitol Drug Resist 4:95 (2014).

Anticancer effects of niclosamide in human glioblastoma. Anja Wieland, Daniel Trageser, Sabine Gogolok, Roman Reinartz, Heike Höfer, Mihaela Keller, Anke Leinhaas, Ramona Schelle, Sabine Normann, Lil Klaas, Andreas Waha, Philipp Koch, Rolf Fimmers, Torsten Pietsch, Anthony T Yachnis, David W Pincus, Dennis A Steindler, Oliver Brüstle, Matthias Simon, Martin Glas and Björn Scheffler. Clin Cancer Res 19:4124 (2013).

Dual-fluorescence isogenic high-content screening for MUC16/CA125 selective agents. Thapi D Rao, Nestor Rosales and David R Spriggs. Mol Cancer Therap 10:1939 (2011).

Lead Optimization of Antimalarial Propafenone Analogues. David Lowes, Anupam Pradhan, Lalitha V Iyer, Toufan Parman, Jason Gow, Fangyi Zhu, Anna Furimsky, Andrew Lemoff, W Armand Guiguemde, Martina Sigal, Julie A Clark, Emily Wilson, Liang Tang, Michele C Connelly, Joseph L DeRisi, Dennis E Kyle, Jon Mirsalis and R Kiplin Guy. J Med Chem 55:6087 (2012).

Optimization of propafenone analogues as antimalarial leads. David J Lowes, W Armand Guiguemde, Michele C Connelly, Fangyi Zhu, Martina S Sigal, Julie A Clark, Andrew S Lemoff, Joseph L Derisi, Emily B Wilson and R Kiplin Guy. J Med Chem 54:7477 (2011).